DNA duplex recognition activates Exo1 nuclease activity

Duplex Stability & Nuclease Resistance

Normalization of genomic DNA using duplex-specific nuclease.

An application of duplex-specific nuclease (DSN) normalization technology to whole-genome shotgun sequencing of genomes with a large proportion of repetitive DNA is described. The method uses a thermostable DSN from the Kamchatka crab that specifically hydrolyzes dsDNA. In model experiments on human genomic DNA, we demonstrated that DSN normalization of double-stranded DNA formed during C0t ana...

Sensitive detection of DNA methyltransferase activity by transcription-mediated duplex-specific nuclease-assisted cyclic signal amplification.

We develop a sensitive fluorescence method for DNA methyltransferase (MTase) assay based on T7 RNA polymerase-mediated transcription amplification and duplex-specific nuclease (DSN)-assisted cyclic signal amplification. This method exhibits excellent specificity and high sensitivity with a detection limit of 0.015 U mL(-1), and it may be further applied for the screening of antimicrobial drugs.

Synthesis, Characterization, DNA Binding and Nuclease Activity of Cobalt(II) Complexes of Isonicotinoyl Hydrazones

Cobalt(II)  complexes of isonicotinoyl hydrazones of two series of ligands have been synthesized and characterized on the basis of elemental analyses, molar conductance, magnetic moment, mass, IR, UV spectral data. Electrochemical behavior of ligands and complexes has been investigated by using cyclic voltammetry. Cyclic voltammetric studies reveal that the oxidation/reduct...

Mechanism of duplex DNA destabilization by RNA-guided Cas9 nuclease during target interrogation.

The prokaryotic clustered regularly interspaced short palindromic repeats (CRISPR)-associated 9 (Cas9) endonuclease cleaves double-stranded DNA sequences specified by guide RNA molecules and flanked by a protospacer adjacent motif (PAM) and is widely used for genome editing in various organisms. The RNA-programmed Cas9 locates the target site by scanning genomic DNA. We sought to elucidate the ...